不同浓度葡萄糖对MG63细胞增殖和细胞周期的影响

Effects of glucose and high glucose fluctuation on proliferation and cell cycle in MG63 cells

目的:观察不同浓度葡萄糖和高糖波动对具有人成骨细胞表型特征的MG63细胞增殖、细胞周期及凋亡的影响,探讨糖尿病性骨质疏松症的发病机制。方法:用不同浓度葡萄糖(5.5、33.3和5.5、33.3mmol/L交替)分别刺激培养的MG63细胞24h,MTT比色分析法测定细胞增殖,流式细胞仪检测细胞周期及凋亡率。结果:①高糖及高糖波动可抑制MG63细胞的增殖,与高糖组相比,高糖波动组的抑制效应更加明显;②高糖及高糖波动可阻滞MG63细胞周期,使G1期细胞比例增加,S期比例减少,诱导细胞凋亡。高糖波动组作用更加明显。结论:高糖波动可抑制成骨细胞增殖,阻滞其细胞周期,诱导细胞凋亡。推测高糖波动可能通过对成骨细胞增殖及细胞周期、细胞凋亡的影响而参与糖尿病性骨质疏松症的发生发展。

Objective：To observe the regulative effect of glucose and high glucose fluctuation on the proliferation,cell cycle and apoptosis in osteosarcoma MG63 cells,and study the role of non-physiological concentration of glucose in pathogenesis of diabetic osteoporosis.Methods：MG63 cells were incubated for 24 h respectively with different concentration glucose（5.5,33.3 mmol /L）and high glucose fluctuation（5.5,33.3 mmol/L）.The proliferation of MG63 cells was examined by MTT colorimetric analysis.The cell cycle of the cells and apoptotic rate were determined by flow cytometry.Results：①Both high glucose and high glucose fluctuation inhibited the proliferation of MG63 cells,and the inhibition effect of high glucose fluctuation group was significantly higher than that of high glucose group（P 〈 0.05）.②Both high glucose and high glucose fluctuation blocked cell cycle,increased the percentage of G1 phase,decreased the percentage of S phase and induced apoptosis.The effect of high glucose fluctuation group was obviously stronger than that in high glucose（P 〈 0.05）.Conclusion：High glucose fluctuation could not only inbihit the proliferation but also block cell cycle and induce apoptosis in MG63 cells,which may be one of the key pathogenetic factors of diabetic osteoporosis.