强脉冲光对小鼠皮肤胶原及PCNA表达的影响

Effects of intense pulsed light on collagens and PCNA expression in mouse skin

目的:观察强脉冲光(intense pulsed light,IPL)对小鼠皮肤的胶原纤维及细胞增殖核抗原(proliferating cell nuclear antigen,PCNA)的影响,探讨强脉冲光嫩肤的分子生物学机制。方法:采用能量密度为32J/cm2强脉冲光照射小鼠背部右侧皮肤,左侧为对照,分别于照射后1、7、14、28天切取皮肤样本,行HE染色,苦味酸-酸性品红染色(VG染色)观察皮肤组织及胶原变化,免疫组化检测皮肤PCNA的表达。结果:与对照部位比较,IPL照射对真皮厚度无明显改变(P<0.05);照射后1、7天两侧皮肤胶原含量无明显改变(P>0.05),14及28天后照射部位皮肤胶原明显增加(P<0.05);PCNA表达于照射后1、7天无明显改变(P>0.05),14及28天显著增强(P<0.05)。结论:强脉冲光可促进皮肤胶原含量增加,并可诱导PCNA表达增强。

Objective：To observe the effect of intense pulsed light（IPL） on collagens and PCNA expressions in mouse skin and elucidate the biological mechanisms of photorejuvenation.Methods：Mice were irradiated on their right sides of the back with IPL（32 J /cm^2）,and their left sides were selected as control areas.At day 1,7,14 and 28 after treatment,skin specimens were taken at treated and non-treated areas,respectively.Histological and collagens changes were observed by hematoxylin-eosin-staining and Van Gieson staining.In addition,the expression of PCNA was investigated by immunohistochemistry at day 28 after irradiation.Results：Compared with non-treated regions,dermal thickness of treated regions had no significant change at day 1,7,14 and 28（P 〉 0.05）.Collagens staining were stronger in treated regions than in non-treated areas at day 14 and 28（P 〈 0.05）.The expression of PCNA also increased at day 14 and 28 after irradiation in treated areas（P 〈 0.05）.Conclusion：IPL can enhance the content of collagens and also can increase the expression of PCNA in mouse skin.