子痫前期患者血清VEGF、TNF-α和sFlt-1水平的变化及意义

Changes and significance of serum VEGF, TNF-α and sFlt-1 in preeclampsia pregnant women

目的探讨子痫前期患者血清中血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)及其可溶性血管内皮生长因子受体(lsFlt-1)的水平及意义。方法采用酶联免疫吸附试验法测定30例重度子痫前期疾病患者血清VEGF、TNF-α及其sFlt-1水平,并与正常孕妇30例对照比较。结果重度子痫前期患者血清VEGF水平显著低于对照组,而血清TNF-α、sFlt-1水平明显高于对照组,差异均有统计学意义(均P<0.05)。重度子痫前期患者血清VEGF水平与TNF-α及sFlt-1均呈负相关(均P<0.05)。结论VEGF、TNF-α、sFlt-1参与子痫前期的发病,VEGF与TNF-α、sFlt-1的相互调节在子痫前期的发病机制中可能起一定作用。

【Objective】 To research the significance and level of serum VEGF, TNF -αand sFlt -1 in preeclampsia pregnant women.【Methods】To adopt the way of the enzyme-linked immunosorbent assay （ELISA） to test 30 cases of patients with severe preeclampsia and 30 cases of healthy pregnant women in serum VEGF, TNF- αand sFlt-1 level , and to make their comparison. 【Results】Maternal serum levels of VEGF in women with severe preeclampsia （SPE） was significantly lower than controls （P 0.05）. The serum levels of TNF-α and sFlt-1 in women with SPE were obviously higher than controls （both P 0.05）. There was significant negative correlation between both TNF-α and sFlt-1 and VEGF （both P 0.05）. 【Conclusion】VEGF, TNF-α and sFlt-1 may contribute to the pathology of preeclampsia, VEGF may play a role in the pathogenesis of preeclampsia by the regulation of sFlt-1 and TNF-α.