RP-HPLC法测定傣药木莲树皮中厚朴酚的含量

Determination of Magnolol in bark of Mulian by RP-HPLC

目的：建立HPLC法测定傣药木莲（Manglietia fordiana Oliv．）树皮中厚朴酚的含量。方法：Zircbrom C18（200×4．6mm，5μ），乙腈-0．5％磷酸溶液（50：50）为流动相，流速1mL·min^-1，检测波长290nm，柱温：30℃，外标定量法。结果：厚朴酚在0．05896—5．896μg范围内有良好线性关系，r=0．9999；样品溶液在24h内稳定，日内精密度良好，RSD=0．82％；平均回收率为98．28％，RSD为3．3％（n=9）。结论：本方法测定木莲树皮中厚朴酚的含量专属性强，精密度好，结果准确可靠。

Objective： To develop an RP - HPLC method to determiine content of Magnolol in bark of Mulina （ Manglietiafordiana Oliv. ）.Methods： RP-HPLC method and irchrom Cls （200 × 4.6mm,5μn） was used. The mobile phase consisted of CH3CN - 0.5 % H3PO4 solution （50： 50） and the flow rate was 1.0mL·min^-1. The UV detection wavelength was 290nm. Column temperature：30℃. Results：The linear range of determination of Magnolol was 0.05896 - 5. 896μg, r = 0. 9999; the sample solution was stable within 24h, the precision in a day was good, RSD = 0.82 % ; the average recovery results of Magnolol were 92.28%, RSD 为 3.3% （n = 9）. Conclusion： This methods was good specialty and precision to determine content of Magnolol in bark of Mulina, rsults were accurate and credible.