乌司他丁对急性重症胆管炎大鼠TNF-α、IL-10的影响研究

目的观察乌司他丁对急性重症胆管炎大鼠TNF-α、IL-10的影响。方法制备急性重症胆管炎大鼠模型,分别在8h及24h两个时间点取血,采用ELISA方法测定血清中TNF-α、IL-10的水平。结果假手术组与正常对照组血清中TNF-α、IL-10没有差异(P>0.05),模型组中8h与24h血清中TNF-α、IL-10较之假手术组及正常对照组明显升高(P<0.05),乌司他丁组中8h,24h血清中TNF-α较之模型组明显降低(P<0.05),8h,24h血清中IL-10较之模型组明显升高(P<0.05)。结论乌司他丁能够通过调节TNF-α、IL-10在血清中的水平发挥免疫调理作用。

Objective Observe the influence of Ulinastatin to acute cholangitis of severe type rat TNF-α and IL-10.Methods Preparation of rat model of severe acute cholangitis,8h and 24h,respectively two points in time to take blood,were measured using ELISA in serum TNF-α and IL10 levels.Result Sham-operated group and the normal control group,serum TNF-α,IL-10 no significant difference （P0.05）.In model group,8h and 24h of serum TNF-α,IL-10 compared with sham-operated group and the normal control group higher （P0.05）.Ulinastatin group,8h,24h of serum TNF-α compared with model group was significantly lower （P0.05）,8h,24h of serum IL-10 compared with model group was significantly higher （P0.05）.Conclusion Ulinastatin able to regulate TNF-α and IL-10 levels in serum play a role in immune conditioning.