固定化L-天冬酰胺酶间歇催化反应的动力学模型

Studies on Kinetics Model of Immobilized Lasparaginase in Batch Reaction System

测定了３７°Ｃ下以固定化Ｌ－天冬酰胺酶为催化剂，５０μｍｏｌ／ＬＬ－天冬酰胺的Ｔｒｉｓ缓冲液（ｐＨ＝７．４）为底物的时歇反应转化率，并用建立的数学模型对反应过程进行拟合。计算结果表明，提出的传质－催化反应动力学模型能较好地描述反应过程，并揭示固定化酶的传质－催化过程受扩散作用控制。

Immobilized Lasparaginase is selected as the studied object. The conversion rate￣elapsed time curve is obtained at 37C in batch and catalytic reaction, with 50mol/L Lasparagine in Tris buffer solution (pH=7.4) as the reaction substrate. The mathematical model is used to simulate the whole reaction process. The calculation results from the models demonstrate that the suggested mass transfercatalysis reaction kinetics models are in good agreement with the actual situation and the whole processes are controlled by diffusion effects.