乌龟脑和肝组织细胞的体外培养

Brain and Liver Tissue Cell Cultures In vitro from Chinemys reevesii

以乌龟脑和肝组织为材料,用φ=20%胎牛血清的DMEM培养液进行体外培养,同时也对肾、心肌、脾脏、尾部肌肉等组织进行了体外培养,结果表明:原代培养的脑细胞(TBCs)和肝细胞(TLCs)生长良好,传代TBCs前3代生长稳定,第4代出现聚集生长,第5代出现"自发转化"现象,至12代,"转化"后的细胞体系生长旺盛;TLCs传代后不易增殖,第3代出现衰退;肾脏细胞在培养过程中有少量贴壁;心肌、脾脏、尾部肌肉的细胞培养都不成功。

The tissues of brain and liver of Chinemys reevesii were cultured in vitro by DMEM contained 20% blood serum of cattle fetus and the kidney,cardiac muscle,spleen and tail muscle also were cultured in vitro.The results showed that the primary culture of the brain cells（TBCs）and the liver cells（TLCs）grew well and grew steadily for 3 generations.The 4th generation of TBCs showed gathering growth,and ＂spontenous transformation＂ occurred in the 5th generation of TBCs.The ＂transformed＂ TBCs grows well until the 12th generation.TLCs propagated hardly after subculture and became declined at the 3rd generation.Some kidney cells attached to the culture plates.The cell cultures of cardiac muscle,spleen and tail muscle were unsuccessful.