胎盘感染小鼠巨细胞病毒对孕鼠脾淋巴细胞增殖反应的影响

Effect of splenic lymphocyte cell proliferation in duration of pregnancy infection by murine cytomegalovirus

目的:观察小鼠巨细胞病毒(MCMV)胎盘感染后孕鼠脾淋巴细胞增殖反应的变化。方法:用ELISA法筛选无MCMV感染史性成熟期小鼠,雌雄同笼受孕。孕龄12.5天时,模型组胎盘显微注射MCMV,对照组注射细胞维持液。在孕龄18.5天处死母鼠。通过正交实验筛选MTT实验最佳条件,以MTT比色法检测小鼠脾淋巴细胞刺激指数。结果:对照组刺激指数(1.395±0.064),模型组刺激指数(0.975±0.029),两组差异具有统计学意义(P<0.01);两组变异系数分别为4.6%及2.9%,均小于5%。结论:MTT法是评价脾淋巴细胞增殖反应的稳定方法。胎盘感染MCMV明显降低孕鼠脾淋巴细胞的增殖活力。

Objective：To study the splenic lymphocyte cell proliferation after placental murine cytomegalovirus （MCMV） infection.Methods：The mature mice without MCMV infection identified by enzyme linked immunoabsorbent assay （ELISA） were served as the subjects.The male and the female mice were putted in the same cages,and then MCMV were injected into the placentas of the model group mice at day 12.5 of gestation,the control group were injected with cell maintenance medium,executed female mice at day 18.5 of gestation;Screened the optimum condition of MTT Assay by direct cross design of experiment,the effect of MCMV to splenic lymphocyte cell proliferation （SI） were observed in duration by MTT Assay in vitro.Results：The stimulation index of the mold group was 1.395 ± 0.064,the control group was 0.975 ± 0.029,the difference between the two groups was significant （P 0.01） ;The coefficient of variation of the two groups was 4.6% and 2.9%,both were smaller than 5%.Conclusion：MTT assay is a stable method to detect splenic lymphocyte cell proliferation.MCMV obviously cuts down splenic lymphocyte cell proliferation in duration of pregnancy placental infection by MCMV.