PTEN与VEGF蛋白在原发性卵巢癌中的表达及临床病理学意义

Study on the expression and clinicopathological significance of PTEN and VEGF in the epithelial ovarian cancer

目的:探讨PTEN、VEGF蛋白表达在原发性上皮性卵巢癌发生、发展、转移中的作用及临床病理学意义。方法:利用SP法对正常卵巢(32例)、卵巢良性肿瘤(31例)、上皮性卵巢癌(74例)PTEN、VEGF基因蛋白的表达进行检测。结果:①PTEN在上皮性卵巢癌表达缺失率及VEGF表达率分别高于对照组,P<0.01;②PTEN在浆液性囊腺癌表达缺失率高于黏液性囊腺癌,P<0.05,晚期表达缺失率高于早期,P<0.01,G3表达缺失率高于G1+G2,P<0.05;③VEGF晚期的阳性率高于早期,P<0.05,淋巴结转移阳性组高于阴性组,P<0.05;④PTEN蛋白表达与VEGF表达不相关。结论:PTEN表达缺失、VEGF蛋白表达在上皮性卵巢癌的发生、发展、侵袭中有重要作用;PTEN、VEGF同时发生异常可作为评估卵巢癌恶性程度的重要生物学指标。

Objective：To investigate the expressions and clinicopathological significance of PTEN and VEGF in the occurance,development and metastasis of primary epithelial ovarian cancer.Methods：74 patients,for controls,31 benign epithelial ovarian tumor and 32 normal ovarian tissue were involved,and their expressions of PTEN and VEGF were detected by immunohistochemical staining.Results：①The negative rate of PTEN and the positive rate of VEGF in epithelial ovarian cancer were higher than that of controls,respectively （P 0.01）.②And the negative rate of PTEN in serous cystadenocarcinoma,advanced stage and G3 period was higher than that of mucous cystoadenocarcinoma,earlier stage and G1 ＋ G2 period clinical stage and histological grade （P 0.05,P 0.01,P 0.05,respectively）.③ The positive rate of VEGF in advanced stage and the group with lymph node metastasis was higher than that of earlier stage and the group without lymph node metastasis （P 0.05,P 0.05,respectively）.④The expression of PTEN was not related with VEGF.Conclusion：The expression deletion of PTEN and the expression of VEGF are the important indicators to assess ovarian cancer occurring,developing and invasion ;The abnormal expression of PTEN and VEGF is very important in assessing malignant behavior of epithelial ovarian cancer as a biological indicator.