基于SRAP分子标记的栽培种花生遗传连锁图谱构建

Construction of genetic linkage map of peanut (Arachis hypogaea L.) based on SRAP markers

采用新型分子标记SRAP（sequence-related amplified polymorphism）技术,以杂交组合（豫花4号×郑8903）的F2群体为材料构建花生栽培种的分子遗传连锁图谱。采用238对SRAP引物对豫花4号和郑8903进行多态性筛选,结果表明用SRAP引物能充分反映两亲本之间的多态性,每个引物组合可产生10~30个左右清晰可辨的条带。筛选多态性较好的78对引物对其F2群体进行分析,共得到287条多态性条带,每对引物组合产生的多态性条带从1~9条不等,平均产生3.68个多态性条带。采用Mapmaker/EXP3.0软件对产生的287个标记位点构建连锁群（LOD≥3.0）,共223个标记位点进入到22个连锁群中,总长2 129.4cM,标记间平均间距为9.55cM。标记在整个连锁群中分布相对比较均匀,这是目前首张基于SRAP分子标记建立的花生栽培种遗传连锁图谱。

A genetic linkage map of cultivated peanut（Arachis hypogaea L.） based on SRAP（sequence-related amplified polymorphism）marker was constructed using F2 population derived from cross Yuhua No.4×Zheng 8903.High polymorphism between the two parental lines was detected with 238 primer combinations.About 10 to 30 clearly distinguishable bands were found for each primer combination.78 primer combinations with high polymorphism were used to screen the F2 population,and a total of 287 polymorphic bands were obtained with an average of 3.68 polymorphic bands per primer pair.A SRAP linkage map was constructed with Mapmaker/EXP 3.0,which contains 223 loci located in 22 linkage groups.The linkage map was 2 129.4cM in length with an average distance of 9.55cM between adjacent markers.All the markers distributed evenly among the linkage groups.This had been the first SRAP-based linkage map for cultivated peanut（A.hypogaea）.