Alcalase碱性蛋白酶水解厚壳贻贝蛋白

Study on Hydrolysis of Mytilus coruscus Protein with Alcalase Protease

以厚壳贻贝为原料,Alcalase2.4L碱性蛋白酶为酶源,在单因素试验的基础上,以还原力A700为指标,运用响应面分析法(RSM)得到Alcalase2.4L酶解厚壳贻贝蛋白的优化条件为:底物浓度[S]3.2%、[E]/[S]2.12%、温度55.54℃、pH8.66、时间2h,该条件下制备的酶解物的还原能力为0.587。同时证明了该酶解物具有显著清除DPPH自由基活性和较强的超氧阴离子自由基清除能力。

Alcalase2.4L were applied to hydrolyze the Mytilus coruscus protein. Based on the results of single- factor tests, reducing power AT00 was chosen as the response values. Using Response Surface Methodology （RSM） , the optimum enzyme hydrolysis conditions of Alcalase2.4L to achieve the maximum antioxidant ability were as follows ： substrate concentration （ [ S] ） 3.20%, [ E ]/[ S]2. 12%, enzymolysis temperature 55.54℃, and enzymolysis time 2 hours. Under such conditions, reducibility of the hydrolysate reached 0. 587, which also had strong scavenging activity of DPPH radical and superoxide anion radical.