摘要
根据文献报道的序列有选择地合成了２０对ＳＳＲ引物，在用以遗传作图的两个亲本中进行了扩增，有１７对引物的扩增产物具有多态性，大小范围为７０—３００ｂｐ。其中扩增产物大小差异小于１０—２０ｂｐ的等位基因可通过４％琼脂糖凝胶电泳分开。介绍了多次点样和多引物ＰＣＲ技术。ｘ２显著性检验证明其符合孟德尔式遗传方式。
Twenty pairs of soybean SSR primers were synthesized according to published data,and 17 out of them have polymorphic alleles in the parents of mapping population, whose sizes are between 70-300bp. Multi liading and multi primer PCR are introduced in this paper. x 2 test shows that microsatellite markers agree with Mendelian model.
出处
《高技术通讯》
EI
CAS
CSCD
1999年第6期8-11,共4页
Chinese High Technology Letters
基金
国家自然科学基金
国家863计划
