小鼠放射性肺损伤动物模型的建立与鉴定

Establishment and indentification of mice model of radtion-induced lung injury

目的建立放射性肺损伤小鼠模型和有效的定量评价方法。方法雌性昆明种小鼠72只,完全随机分为对照组(18只)、照射组(54只)。全肺单次照射20Gy,于照射后1、3d和1、2、4、8周取照射组及相同鼠龄对照组小鼠肺组织和静脉血,分别用HE、Masson染色,在光镜下观察组织病理改变和胶原纤维沉积情况,用酶联免疫(ELISA)法测定血清转化生长因子-β1(TGF-β1)含量。结果与对照组比较,照射组小鼠肺组织早期以渗出为主,表现为肺间质及肺毛细血管充血、水肿,照射后期以肺泡间隔及部分肺泡腔内可见纤维素样渗出物为特征。同时,ELISA法显示照射后7d血清TGF-β1含量[(18.46±1.678)ng/mL]明显增高,与对照组[(9.61±2.027)ng/mL]比较差异有统计学意义(P<0.01)。结论通过病理形态学和血清学指标证实成功地建立了放射性肺损伤小鼠模型,该模型较好地模拟了放射性肺损伤的发生、发展过程。

Objective To establish a mice model of radiation-induced lung injury and effective quantitative assessment methods.Methods Seventy-two Kunming female mice were assigned randomly to control group（recevied sham-irradiation,n=18）and treatment group（recevied irradition on the thoraces with single fraction 20-gray X-rays,n=54）.The mice were sacrificed at 1,3 days and 1,2,4,7 weeks after the irradiation,lung tissues and venous blood were extracted to observe the pathological changes collagen fiber deposition of lung tissue by HE and Massion staining,and measure the content of TGF-β1 in blood serum via ELISA methods.Results Compared with the control group,significant effusion eg.vasocongestion,edema of lung interstitial substance and pulmonary capillary was observed in the treatment group at early stage after irradiation.At late stage after irradiation,cellulose effusion was main in alveolar septum and alveolar space.The difference of TGF-β1 content was significant（P0.01）between treatment group（18.46±1.678）ng/mL and control group（9.61±2.027）ng/mL at 7 days after irradiation by ELISA method.Conclusion A mice model of radition-induced lung injury that was confirmed by the pathological morphology and blood serum index was established succussfully.