摘要
人角质细胞生长因子(Keratinocyte growth factor,KGF)在组织的损伤修复中起着重要的作用.植物细胞生物反应器是一种成本低、安全性好的蛋白生产系统.本研究将人KGF-1经农杆菌介导法转入水稻中,共获得38株抗性再生植株.Southern杂交和RT-PCR结果表明KGF-1基因整合入水稻基因组中且部分转化株中KGF-1基因得到转录.Western blotting检测结果显示有两株转化水稻中能够表达KGF-1.
Keratinocyte growth factor（KGF） plays an important role in the tissue damage repair.Plant cell bioreactor is a low cost and safe protein production system.Human KGF-1 gene was transformed into rice by Agrobacterium-mediated method and a total of 38 putative transgenic rice plants were obainted.The results of southern blotting and RT-PCR assay showed that KGF-1 gene was integrated into the rice genome and KGF-1 gene was transcribed in part of the putative transformed rices.Western blotting results showed that KGF-1 was expressed in two transgenic rices.
出处
《漳州师范学院学报（自然科学版）》
2010年第3期97-100,共4页
Journal of ZhangZhou Teachers College（Natural Science)
基金
福建省自然科学基金资助项目(2010J01240)
关键词
角质细胞生长因子
水稻遗传转化
基因表达
keratinocyte growth factor-1
rice genetic transformation
gene expression
