5-氮胞苷和利福平对丛枝病泡桐幼苗形态和蛋白质变化的协同影响

Effects of 5-Aza and Other Extraneous Substances on the Changes of Morphology and Protein of Paulownia Seedlings with witche’s Broom

以感染丛枝病的豫杂一号泡桐幼苗为试验材料,通过不同浓度5-氮胞苷和利福平处理,研究了其幼苗形态和蛋白质的变化。结果表明,适宜浓度的利福平和5-氮胞苷共同使用可抑制泡桐丛枝病的发生。150μmol·L-15-氮胞苷+80 mg·L-1利福平可以抑制豫杂一号泡桐丛枝病症状。存在于健康豫杂一号幼苗中的蛋白质pI 6.8、m24ku而在病苗中观察不到。表明该蛋白质可能与豫杂一号泡桐丛枝病发生有一定关系。

The paper take the seedlings of p.tomentosa×p.fortunei paulownia infected witche’s broom as the experiment material.Reaserch on the effect on the morphological and the preotin change that treated by different concentration of 5-Aza and rifampin.The main results are as following： Appropriate concentration of 5-Aza and rifampin can restrain paulownia witche’s broom.Treament of 150 μmol·L-15-Aza＋80 mg·L-1 rifampin can restrain paulownia witche’s broom.The protein（pI 6.8、m24ku） exist in the healthy seedlings can not be observed in the seedlings of witche’s broom.This indicate that the protein possibly has some realationship with paulownia witche’s broom.