长期冻存对昆虫细胞系SL2和NIH-SaPe-4活性的影响

Effects of Long-term Cryopreservation on the Cytoactive of Two Insect Cell Lines SL2 and NIH-SaPe-4

对2种双翅目昆虫细胞系(黑腹果蝇胚细胞系SL2以及麻蝇胚细胞系NIH-SaPe-4)38个月长期液氮深低温保存效果进行了研究,通过对冻存时间和保护剂对2种细胞系冻后活力、圆度以及恢复时间影响的测定,比较了3种不同配方冻存保护液的效果。结果表明:2种昆虫细胞系冻后活性均随着冻存时间的增长而逐渐降低。长期冻存期中,使用90%胎牛血清(FBS)+10%二甲基亚砜(DMSO)冻存SL2效果较好,NIH-SaPe-4使用10%DMSO即可满足冻存的需要,而无需额外添加高浓度FBS。甘油不适用于这2种昆虫细胞系的长期保存。

In order to evaluate the effects of different cryoprotectants and durations on post-thaw cytoactives（including cell viability,circularity and recovery time） of two dipteral insect cell lines,3 kinds of cryoprotectants（Formula I： 10% DMSO ＋ 90% FBS;Formula II： 10% DMSO;Formula III： 10% glycerol） were used to preserve the cells of SL2 from embryo of Drosophila melanogaster and NIH-SaPe-4 from embryo of Sarcophaga peregrine in liquid nitrogen separately.The results showed that the post-thaw viability of these two cell lines decreased with the cryopreservation time expended.In long-term cryopreservation,the effect of Formula I for preserving SL2 cells was better than the others.Formula II was suitable for NIH-SaPe-4.Using glycerol as cryoprotectant was unsuitable for these two insect cell lines.