自然侵染白肋烟的黄瓜花叶病毒的鉴定

IDENTIFICATION AND CHARACTERIZATION OF CUCUMBER MOSAIC VIRUS ISOLATES NATURALLY INFECTING TOBACCO VAR.WHITE BURLEY

根据１９９５ ～１９９６ 田间调查表明，ＣＭＶ 是侵染白肋烟的主要病毒之一。采集到一种（ 命名为ＣＭＶ－ ＴＢ） 引起烟株花叶、植株矮化的样品，摩擦接种能侵染７ 科２９ 种植物。ＣＭＶ－ ＴＢ 引起苋色藜局部枯死斑。ＣＭＶ－ ＴＢ 体外稳定性状：体外保毒期２ 天；稀释限点１０ － １ ；致死温度５０ － ５５ ℃。电镜观察ＣＭＶ－ ＴＢ 为球状颗粒，直径３０ｎｍ 。ＳＤＳ聚丙酰胺电泳测其分子量２８ ８００Ｄ。ＣＭＶ－ ＴＢ 和ＣＭＶ－ ＣＡ（ 花生株系） 抗血清起强阳性反应，与同一病毒组的花生矮化病毒（ＰＳＶ） 抗血清起阴性反应。基于以上性质，ＣＭＶ－ ＴＢ 经鉴定属于黄瓜花叶病毒。本研究为侵染白肋烟的ＣＭＶ 国内首次详细报道。通过应用生物学（ 寄主反应和温度敏感性） 实验与ＣＭＶ－ Ｓ、ＣＭＶ－ Ｄ 对比，参试的分离物在３２ ℃下引起昆诺藜（ Ｃ．ｑｉｎｏａ） 、豇豆（Ｖ．ｓｉｅｓｉｓ） 产生局部枯斑并且与ＣＭＶ－ Ｄ 抗血清产生比ＣＭＶ－ Ｓ抗血清更强的特异性血清学反应。以上实验证明，ＣＭＶ－ ＴＢ 中国分离物与ＣＭＶ－ Ｄ 性质相同，属于ＣＭＶ 亚组Ｉ，而与ＣＭＶ 亚组ＩＩ有明显差异。依据在一组寄主植物上的反应，对参试的ＣＭＶ－ ＴＢ

The results of surveys made in 1995～1996 showed that the virus of white Burley tobacoo was mostly caused by cucumber virus ,CMV-TB (Tobacoo var.White Burley Isolate) causes leaf mosaic and stunting on tobacoo var.White Burley and infected 29 species of plants inoculated in the greenhouse .CMV-TB causes local necrotic spots on inoculated leaves of Chenopodium amaranticolor .In vitro stability of CMV-TB is as follows:dillution end point,10 -1 ;thermal inactivation point at 50～55℃;longevity in vitro,2 days.CMV-TB is isometric with diameter of 30.0nm .Molecular weight of coat protein is 28 800 D by electropheresis in polyacrylamide gels.CMV-TB is closely related to CMV-CA(peanut isolate) ,and distant to Psv-Mi serologically. Based on the above properties CMV-TB is indentified as CMV. CMV-TB infecting tobacco.var White Burley is first reported in detail. CMV-TB local necrotic lessions on inoculated leaves of Chenopodium qinoa and Vigna sinesis in 32℃ and is closely related to CMV-D(type strain in CMV subgroup I),distinct to CMV-S(type strain in CMV subgroup II)serologically,which indicates CMV-TB belongs to CMV subgroup I.Based on the host reactions on a group of the indicative plants the strain designation is proposed.