缺氧性肺动脉高压大鼠的降钙素基因相关肽含量和分布

The content and distribution of CGRP in rats with hypoxic pulmonary hypertention

目的：研究降钙素基因相关肽（ Ｃ Ｇ Ｒ Ｐ） 对缺氧性肺动脉高压（ Ｈ Ｐ Ｈ） 的影响。方法：应用常压缺氧方法建立大鼠肺动脉高压动物模型；采用酶联免疫测定法测定大鼠血浆降钙素基因相关肽的含量；用免疫组织化学染色法观察肺组织中 Ｃ Ｇ Ｒ Ｐ 的分布。结果：缺氧组大鼠血浆 Ｃ Ｇ Ｒ Ｐ 含量较正常对照组有明显下降（ Ｐ＜ ００１） ，且血浆 Ｃ Ｇ Ｒ Ｐ 水平与肺动脉平均压（ ｍ Ｐ Ａ Ｐ） 呈负相关（ Ｐ＜００５） ；正常大鼠肺组织有极少量 Ｃ Ｇ Ｒ Ｐ 样免疫物质，缺氧组大鼠肺组织有大量 Ｃ Ｇ Ｒ Ｐ 样免疫物质。结论：缺氧时血浆 Ｃ Ｇ Ｒ Ｐ 含量减少可能是其释放减少所致， Ｃ Ｇ Ｒ Ｐ 在缺氧继发肺动脉高血压中起重要的肺循环调节作用。

Aim:To study the influence of CGRP on hypoxic pulmonary hypertention (HPH). Methods: A stable rat model of HPH was established by chronic normobaric hypoxia. The plasma CGRP content was measured by enzyme immunoassay. The CGRP-like immunoreactivity in the rats′ lung tissue was observed by immunohistochemical staining as using labelled streptavidin biotin (LSAB). Results: The plasma CGRP content of hypoxic grpup was very much lower than the control group ( P <0 01). The relative coefficient between CGRP and mPAP is-0 717 ( P <0 05). The CGRP-like immunoreactivity was mostly localized around pulmonary vessels and pulmonary interstitium in hypoxic group, contrasted sharply with control group in which there were little CGRP staining. Conclusion: Those results implyed that hypoxia could reduce CGRP release, which caused the decrease of plasma CGRP content. CGRP plays on important role in regulating the resistance of pulmonary circulation and has close relation with the formation of HPH.