亚硝化对尼古丁基因毒性影响的研究

Effect of Nitrosation on the Genotoxicity of Nicotine

引言流行病学调查结果显示［１］，吸烟是造成肺癌、喉癌、口腔癌等多种癌症的重要因素；Ｐｈｉｌｉｐｓ等［２］研究发现吸烟者肺细胞中的ＤＮＡ加合物与其每天吸烟支数线性相关。尼古丁是烟草中最主要的生物碱，占烟草重量的１％～２％，是使吸烟成瘾的决定性因素，因此...

Whether nicotine undergoes in vivo nitrosation transforming carcinogens is s till a pending problem.We report the effect of nitrosation on DNA adduction with nicotine in vivo and in vitro studied by accelerator mass spectrometry( AMS) and liquid scintillation counting technique,using 14 C labeled nic otine as a tracer.Our results show that under simulated acidic gastric condition and other proper conditions,nicotine could be nitrosated,thus enhancing its gen otoxicity.But this process significantly depends on the dose level of nitrite.Wh en NaNO 2 dose lowers to in vivo level,the nitrosation process is too weak to be detected.When NaNO 2 dose raises,it increases the formation of DNA adduct s,thus enhancing the genotoxicity of nicotine.