基因芯片法检测抗高血压药物相关基因多态性

Detection of polymorphisms in antihypertensive drug related genes by gene microarray

目的检测中国人的5个抗高血压药物相关基因的多态位点,进行基因分型,计算检测位点的等位基因频率,为预测遗传因素对治疗高血压药物的影响提供数据。方法用基因芯片法对220例样本的5个抗高血压药物相关的基因多态位点进行检测分型。结果检测了220例样本基因型,计算得到4个等位基因频率,CYP2C9*3为5.68%,ADRB1(G1165C)C为74.09%,AGTR1(A1166C)C为7.73%,ACE(I/D)等位基因D为34.09%。有6例样本CYP2D6基因检测无结果。结论 5个抗高血压药物相关多态位点在220例中国人中均有分布,可能影响相关药物在个体中的作用,从而影响高血压病的疗效。

Objective To detect and genotype five polymorphisms in antihypertensive drug related genes in Chinese.The frequencies of the alleles were calculated to provide genetic parameters for prediction of the influence in antihypertensive drug treatment.Methods The genotypes of five polymorphisms in antihypertensive drug related genes were detected by gene microarray in 220 samples.Results The frequencies of four polymorphisms were calculated in 220 samples,in which frequency of CYP2C93 was 5.68%,ADRB1 1165C allele was 74.09%,AGTR1 1166C was 7.73%,and ACE D allele was 34.09% respectively.Six samples were no results in detection of CYP2D6 by gene microarray.Conclusion The alleles of five gene polymorphisms were discovery in 220 samples.They maybe affect interaction between body and drug and cause individual variation.