中国人体棘阿米巴角膜炎分离株的形态特征和分类鉴定

Characterization and Identification of Acanthamoeba Keratitis Pathogens from First Human Cases in China

国内首次发现的两例人体棘阿米巴角膜炎样品在作者所在实验室进行了形态学特征分析和致病性实验。这两个分离株（Ｌ和Ｗ）的形态特征极其相似：滋养体平均长度为２７．５μｍ，平均宽度为２１．０μｍ。包囊的平均直径为１３．６０μｍ；外囊贴近内囊，内囊呈多边形，但趋近圆形，内囊臂数为５—７；蛋白银染色显示不存在网状结构。两虫株对小鼠均有致病力。根据以上特征，作者将这两个中国人体致病性虫株初步鉴定为棘阿米巴属类群２中的Ａｃａｎｔｈａｍｏｅｂａｌｕｇｄｕｎｅｎｓｉｓ－Ａ．ｑｕｉｎａｃｏｍｐｌｅｘ．

The pathogenic strains were isolated from two acanthamoeba keratitis patients wearing soft contact lens in China. Two strains (L and W) were axenically cloned in our laboratory. The morphological characters of two Acanthamoeba strains were very similar: the cyst was not reticulate; most endocysts were typically polyhedral, with tendency to be spherical; the arms were broad and not prominent, the mean number of endocyst arms was 5-7; gently wrinkled ectocyst was closely applied to endocyst; the mean cyst diameter was 13.60μm. Both strains were pathogenic to mice. According to the characterization mentioned above these Chinese pathogenic strains were preliminarily identified as a member of Acanthamoeba lugdunensis-quina complex(Group Ⅱ).