红藻藻胆体内部蛋白间的能量传递研究 Ⅲ.人工合成复合物R-PC/APC内的能量传递

Energy Transfer among Proteins in the Phycobilisome of Red Algae Ⅲ.Energy transfer in Synthesized Complex of R PC/APC

研究了人工合成的二元藻胆体模拟复合物（ＲＰＣ／ＡＰＣ）的时间分辨荧光光谱并运用多指数拟合的方法对数据进行了详细的处理和分析，研究结果证实了能量在ＲＰＣ和ＡＰＣ之间的传递时间与能量从ＲＰＥ传递到ＲＰＣ的时间几乎相等（５０ｐｓ）；除此之外，ＲＰＣ到ＡＰＣ没有其它能量传递的通道．结果为能量可以从ＲＰＥ直接传递到ＡＰＣ同时也可以经过ＲＰＣ传递到ＡＰＣ，ＲＰＣ作为能量传递的中间受体，在实际的体系中其作用是通过竞争的机制实现的．

Time resolved fluorescence spectra of artificial synthesized functioning complex of R PC/APC has been studied by multi exponential deconvolution technique in detail The results show that the energy transfer time between neighboring proteins is 50 ps, and improve that there is path for energy transfer from R PE to APC with time constant of 110 ps and this path does not exists between R PC and APC