转多个抗真菌蛋白基因水稻植株的获得及其抗稻瘟病菌的初步研究

Introduction of Multiple Antifungal Protein Genes into Rice and Preliminary Study on Resistance to Pyricularia oryzae of Transgenic Rices

用基因枪法将水稻碱性几丁质酶（ＲＣ２４）基因、苜蓿葡聚糖酶（βＧｌｕ）基因、大麦核糖体失活蛋白（ＢＲＩＰ）基因和潮霉素（ｈｐｔ）基因同时导入籼稻品种（七丝软占）中，获得了７个潮霉素抗性再生系，Ｓｏｕｔｈｅｒｎｂｌｏｔ证明２～３个抗真菌蛋白基因已整合到水稻基因组中．

Three antifungal protein genes: the rice basic chitinase gene, the alfalfa glucanase gene, the barley ribosome inactivating protein gene and a hygromycin phosphotransferase gene were introduced into an indica variety riceQisiruanzhan by biolistic Seven independent lines were obtained by selecting for hygromycin resistance Southern blot proved two or three antifugal protein genes integrated into rice genome The transgenic plants showed an enhanced resistance to Pyricularia oryzae in vitro