有机溶剂中固定化脂肪酶催化水解植物油脂的研究

STUDY ON THE HYDROLYSIS REACTION OF PLANT OIL BY THE IMMOBILIZED LIPASE IN THE ORGANIC SOLVENT

研究了在有机溶剂中固定化脂肪酶对樟核油、棕榈油水解反应的催化作用，考察了有机溶剂／水、固定化酶量、反应时间等因素对水解反应的影响。实验结果表明：在３７℃时，水解樟核油的最佳条件是有机溶剂／水９８％、固定化酶量００２ｇ／ｇ油、反应时间６ｈ，而水解棕榈油的最佳条件是温度３５℃、有机溶剂／水９５％、固定化酶量００２ｇ／ｇ油、反应时间４ｈ。

This paper studied that camphor-kemel oil and palm oil were hydrolyzed by the immobilized lipase in the organic solvent.We approached the effects of the hydrolysis reaction from different water content in organic solvent,different immobilized lipase content,and different reaction time and temperature.The result indicated that the optimum reaction condition of the hydrolysis was separately relative of organic solvent and water 98%,immobilized lipase content 0.02 g/g oil and the reaction time 6 hours for camphor-kemel oil,and that the optimum reaction condition of the hydrolysis was separately temperature 35 ℃,the relative of organic solvent and water 95%,immobilized lipase content 0.02 g/g oil and the reaction time 4 hours for palm oil.