乙酰甲胺磷对斜生栅藻的毒性及细菌降解研究

Study on toxicity of acephate to Scenedesmus obliquus and degradation of bacteria to acephate

采用血球计数法测定斜生栅藻细胞数目,研究了乙酰甲胺磷对斜生栅藻的毒性作用,并通过藻类培养液中接种经过筛选得到的乙酰甲胺磷高效降解菌测定其对乙酰甲胺磷毒性的去除影响。研究结果表明,乙酰甲胺磷对斜生栅藻的抑制中浓度(EC50)大于159 mg/L,急性毒性为低毒。但进一步研究发现,在7 d后,EC50为174.68 mg/L,明显低于24 h的1 128.57 mg/L,存在亚慢性毒性。通过藻类培养液接种高效降解菌Y-6,乙酰甲胺磷对藻类的生长抑制程度减轻。

A study was carried out to determine the toxicity of acephate to the algae,Scenedesmus obliquus,making use of a microscope with counting chamber.And a strain of bacteria designated as Y-6 was added into the solution to evaluate the effect of degrading acephate.It was shown that the median inhibition concentrations（EC50） were more than 159 mg/L in slightly acute toxicity.The further result showed that acephate was subchronic toxicity to algae with the EC50 of 7 d（174.68 mg/L）,which was significantly lower than EC50 of 24 h（1 128.57 mg/L）.When the high-degrading bacteria Y-6 was inoculated into the solution,the inhibition of alage by acephate was eased.