摘要
通过两次离子交换柱层析， 从牛脑中制备钙调素依赖性的环核苷酸磷酸二酯酶． 所得酶不含内源性钙调素， 能被足量外加的钙调素激活 １４ 倍，
Calmodulin dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two step DEAE cellulose column chromatography. The enzyme obtained was free of endogenous calmodulin and stimulated about 14 fold by exogenous calmodulin, it can be used to assay the content of cytosolic calmodulin.
出处
《吉林大学自然科学学报》
CSCD
1999年第3期81-84,共4页
Acta Scientiarum Naturalium Universitatis Jilinensis
关键词
环核苷酸
磷酸二酯酶
钙调素
制备
PDE
cyclic nucleotide phosphodiesterase, calmodulin, preparation
