摘要
目的比较MPTP处理的小鼠与6-OHDA损毁大鼠PD模型的病理变化。方法应用免疫组化方法观察两种PD模型的中脑腹侧多巴胺能神经元,星形胶质细胞的变化。结果免疫组化方法结果表明:C57BL小鼠在MPTP处理后,脑内酪氨酸羟化酶阳性神经元的数目从第4d开始有所减少;黑质区域的GFAP免疫阳性星形胶质细胞数目从第1d起即有增加。大鼠PD模型较早出现损毁侧TH免疫阳性神经元明显减少的现象,损毁2月后几乎完全消失;而在黑质区的GFAP免疫阳性星形胶质细胞数目增多现象出现较晚。结论两种动物模型从不同侧面反映了帕金森病的病理特征,6-OHDA损毁中脑DA能神经元制成的大鼠PD模型比MPTP处理的C57BL小鼠PD模型更接近反映PD的病理变化。
Objective To compare the pathology changes between two kinds of PD animal models with MPTP treated mice and 6 OHDA lesioned rats.Methods Thirty six C 57 BL male mice with 7 weeks old administrated subcutaneously by MPTP(30mg/kg) once every day were divided into different groups,according to their MPTP treated time.Twenty SD rats with unilateral 6 OHDA lesioned dopamine neurons in the midbrain,were used for PD models which were divided into two groups,according to postoperative survival time.The abnormal rotations could be induced by injection of apomorphine in all these rat models.Results The immunohistochemical staining showed that the loss of TH positive neurons did not appear until the 4th day for treating mice with MPTP,while a increase of GFAP positive astrocytes could be seen from lst day of injection.The immunohistochemical staining of rat brain sections showed that a significant loss of TH postive neurons in the lesion side could be observed from early stage,and completely disappeared from 2 months after lesion.The GFAP postive astrocytes in the same field,on the other hand,significantly increased rather late.Conclusion The pathology changes of both mouse and rat models could reflect PD ,s from different aspects,but rat PD model could reflect PD ,s better.
出处
《中风与神经疾病杂志》
CSCD
北大核心
1999年第2期79-82,共4页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金
关键词
酪氨酸羟化酶
震颤性麻痹
6-羟多巴胺
MPTP
Tyrosine hydroxylase Glial fibrillary acidic protein Immunohistochemistry Parkinson
s disease.