大鼠实验性短暂脑缺血血浆中cAMP相对浓度与血脑屏障变化之关系

Moderate hypothermia reduce the mortality and the content of calcium in the brain after experimental cerebral hemorrhage in rats.

目的观察短暂脑缺血再灌流过程中血脑屏障超微结构及血浆中环腺苷酸（ｃＡＭＰ）相对浓度的变化。方法用改良ｐｕｌｓｉｎｅｌｉ法造成短暂脑缺血再灌流模型。用Ｈ３－ｃＡＭＰ放免分析药盒测定血浆中ｃＡＭＰ相对浓度，并观察血脑屏障中紧密连接的变化。结果在短暂缺血及再灌流早期，血浆中ｃＡＭＰ相对浓度及紧密连接紧密程度（闭锁小带长度、凸嵴数目）无明显变化，血管内皮中胞饮小泡数目明显增多。再灌注中期内皮细胞间紧密连接松弛，闭锁小带长度及凸嵴数目均减少。血浆中ｃＡＭＰ浓度亦降低。结论短暂脑缺血及再灌流早期血脑屏障通透性的改变，主要是由于内皮细胞中胞饮小泡数增多所致。血浆中ｃＡＭＰ相对浓度可以反映血脑屏障的开放程度。

Objective To study the effects of moderate hypothermia on the mortality during twenty four hours and the content of calcium in the brain after experimental cerebral hemorrhage in rats.Method Of 134 rats were divided into two parts:(1)68 rats were used for observing of the mortality,(2)66 rats were used for determining the content of calcium in the brain.Every part was divided into three groups:(1)sham operated group,(2)nor morthermic group (37℃),(3)hypothermic group(32℃).Results During 24 hours after cerebral hemorrhage,the mortality is 36.67% in the normothermic group, and it is 4.55% in the hypothermic group.In the normothermic group,the content of calcium is remarkablely highter than that of the sham operated group and the hypothermic group.Conclusion Hypothermic therapy can reduce the mortality and the content of calcium in the brain after experimental cerebral hemorrhage in rats.