白细胞介素-18基因多态性与脑梗死相关性研究

The relationship of interleuldn-18 gene polymorphlsms in patients with cerebral infarction and its effect on plasma lipid levels

目的探讨白细胞介素-18（IL-18）基因启动子区-137G／C与脑梗死关系及其对血脂、脂蛋白水平的影响。方法运用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）方法检测190例脑梗死患者及对照组210例的IL-18基因型，同时测定血浆脂质、脂蛋白水平。结果IL—18基因-137G／C多态性在两组人群中的分布差异具有统计学意义（P〈0．05），等位基因频率的相对风险分析发现，C等位基因携带者患脑梗死的风险是G等位基因的1．624倍（OR=1．624，95％CI：1．134—2．324）；携带C等位基因的脑梗死个体血清TC水平显著高于不携带者（P〈0．05）。结论IL-18基因．137G／C多态性与脑梗死发病具有相关性，其中C等位基因可能是脑梗死的遗传易感基因。

Objective To study the relationship of interleukin-18 （IL-18）gene-137G/C polymorphisms in the promoter region with cerebral infarction（CI） and the effect of integrin gene polymorphisms on plasma lipid and lipoprotein levels. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） were used for the detection of integrin genotypes in 190 patients with CI and 210 healthy controls. The plasma lipid and lipoprotein levels were measured by routine method. Results The distributions of IL-18 gene -137G/C polymorphism was significantly different between CI group and control group（ P 〈 0. 05） ;The relative risk suffered from CI of C allele was 1. 624 times of the G allele（ OR = 1. 624,95% CI： 1. 134 - 2. 324 ）, The level of plasma lipid C allele carriers was significantly higher than no earriers（P 〈0. 05）. Conclusion IL-18 gene-137G/C polymorphism was associated with CI, C allele may be genetic susceptibility gene for CI.