摘要
目的：分析山参遗传多样性及其遗传特性。方法：用随机扩增多态ＤＮＡ（ＲＡＰＤ）标记方法对７个来源地不同的山参和１个园参样品进行遗传多样性检测和遗传分析。结果和结论：用１４个１０ｍｅｒ寡聚核苷酸引物共检测１１１个位点，其中多态位点７６个，占６７６％，远大于园参内的遗传变异，因此山参在人参育种上有很大利用价值。聚类分析表明，山参之间及其与园参之间的遗传变异，没有超出与近缘种西洋参之间的遗传差异；遗传因素在人参形态变异上的作用小于环境因素，这一结果为“山参”的培育提供了理论依据。
AIM: To analyse the genetic
characteristics of wild ginseng. METHODS: The genetic diversity level of wild ginseng (Panax
ginseng C.A.Meyer) was confirmed by random amplified polymorphic DNA (RAPD) markers.
Seven wild ginsengs, collected from different sites, with one garden ginseng were used in the
study. RESULTS and CONCLUSION: From the fourteen 10mer oligonucleotide primers 111
sites were detected, of which 76 (676%) were polymorghic. The level of genetic variation in wild
ginseng was much higher than that in garden ginseng. Therefore, wild ginseng was a very
precious material in breeding. According to the pairwise distances of all samples, one
conclusion could be drawn that the evironment factor plays more important role than genetic
factor in morphological change of wild ginseng which offers an evidence that even garden
ginseng seed can develop to wild ginseng if it is planted in the mountains. Cluster analysis
showed that the genetic variations among P.ginsengs were smaller than that betwe
出处
《药学学报》
CAS
CSCD
北大核心
1999年第4期312-316,共5页
Acta Pharmaceutica Sinica
关键词
人参
野生人参
RAPD
遗传多样性
遗传距离
wild ginseng
random amplified polymorphic DNA (RAPD)
genetic diversityen P.ginsengs and
P.quinquefolius.
