新疆南部维吾尔族人群尿石症与血管内皮生长因子基因多态性关系的研究

Distribution of vascular endothelial growth factor gene polymorphism in Uyghur Ethnic patients with urolithiasis insouth Xinjiang

目的 探讨新疆南部维吾尔族人群血管内皮生长因子（VEGF）-460位点基因多态性与泌尿系结石的关系.方法 应用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）、基因测序等方法检测200例尿石症患者的VEGF-460基因多态性,200例健康人群体检标本作为对照组,比较2组VEGF-460基因型和等位基因的分布频率.结果 2组VEGF-460位点CC、TT、CT基因型和等位基因比较差异均无统计学意义（P＞0.05）,基因型分布频率分别为1.5%、29.0%、69.5%和0.5%、27.5%、72.0%,等位基因C、T分别为36.2%、63.7%和36.9%、63.1%,2组比较差异均无统计学意义（P＞0.05）.结果 新疆南部地区维吾尔族人群VEGF-460基因多态性与尿石症无关,VEGF-460位点可能不是维吾尔族尿石症的易感基因.

Objective To determine vascular endothelial growth factor（VEGF）-460 gene polymorphism in Uyghurs and its relationship to urolithiasis in south Xinjiang. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）,gene sequencing and genetic analysis methods were used in 200 urolithiasis patients of Uyghurs, and 200 healthy Uyghurs. Results The distribution of genotype and allele had no significant difference between urolithiasis patients and normal controls （P〉0. 05）. The frequencies for the CC,TT and CT genotypes in patients with urolithiasis and normal controls were 1.5 %, 29.0 %, 69.5 % and 0. 5 %, 27.5 %, 72.0 %, respectively. The frequencies for C and T allele were 36.2%,63.7% and 36.9% ,63.1%, respectively. Conclusions The results of VEGF-460 gene polymorphisms indicate no significant relationship between patients with turolithiasis and normal controls in Uyghurs in south Xinjiang,which may not be urolithiasis susceptibility genetic locus.