培养条件对细脚拟青霉抑制金黄色葡萄球菌的影响

Effects of liquid culture conditions on inhibition of Paecilomyces tenuipes against Staphylococcus aureus

目的:研究不同培养条件对细脚拟青霉抑制金黄色葡萄球菌的影响,确立最佳摇瓶培养条件。方法:以抑菌圈直径大小为指标,采用单因素实验和L9(34)正交实验,对碳源、氮源、无机盐、培养时间、起始pH、摇瓶装液量以及接种量等培养条件进行研究。结果:细脚拟青霉产抑菌活性物质的最佳培养基为:4%蔗糖、2%酵母浸出粉、0.15%MgSO4.7H2O和0.025%KH2PO4;最佳摇瓶培养条件为:起始pH5.5～6.5,装液量30mL/100mL,接种量5%～7.5%,培养时间6d。结论:在此优化条件下,细脚拟青霉发酵液对金黄色葡萄球的抑菌圈直径可达23.5mm。

Objective：To study the effects of liquid culture conditions on inhibition of Paecilomyces tenuipes against Staphylococcus aureus and get the optimal culture conditions. Methods： Single factor test and L9 （3^4） orthogonal design were used to study culture conditions with the diameter of inhibition zone as index. Results： The optimal culture medium was sucrose 4% ,yeast powder 2% ,MgSO4 · 7H2O 0.15% and KH2PO4 0.025%.The optimal culture conditions were initial pH 5.5-6.5, media volume 30mL/100mL, inocula amount 5%-7.5% and culture time 6d. Conclusion：The diameter of inhibition zone of Staphylococcus aureus could reach 23.5mm under the conditions.