蚕豆根尖微核实验阳性结果判断标准研究

Study on judgment criterion of positive result in Vicia Faba root tips micronucleus test

目的:研究蚕豆根尖微核实验阳性结果判断标准。方法:蚕豆根尖微核实验。结果:用方差分析(t检验)比较各致突变物微核率与对照组微核率的差异显著性,提出当微核指数大于1.5时,可以判定实验结果为阳性,即该物质确有致突变性。

Aim：To study the judgment criterion of positive result in Vicia faba root tips micronucleus test.Methods： Micronucleus test of Vicia faba root tip cells.Conclusions：The analysis of variance （t test）was used to compare the significance of difference between the micro-nucleus rate that measured by these mutagens and the control group, proposing if the micro-nuclear index more than 1.5 ,then it can be judged the result was the positive result, that is to say,the material did lead to mutation.