气相色谱质谱联用法检测牛奶及奶粉中肌醇

Determination of myo-inositol in milk and milk power by gas chromatography and mass spectrometry

利用70%的乙醇从样品中提取肌醇,然后采用硅烷化试剂(三甲基氯硅烷∶六甲基二硅胺烷∶N,N二甲基甲酰胺=1∶2∶8)进行衍生,通过GC/MS进行检测。通过全扫描确定,特征离子的m/z73.0、147.0、191.1、217.1、305.2为定性离子,其中m/z73.0为定量离子。方法线性关系良好,相关系数为0.9987,方法检出限为9μg/100g,RSD分别为7.997%、2.515%、2.529%(n=7),方法的回收率在88.5%～108.1%之间,均能满足样品检测的要求。

The myo-inositol that was extracted by the 70% ethanol from the milk and milk power, and then was trimethylsilylated with the silane agents, which was composed of trimethyl chlorosilane, hexamethyldisilazane and N, N- dimethylformamide （ 1 ： 2： 8 ）, was determined by GC/MS. After full- scan detection, the characteristics ions m/z 73.0,147.0,191.1,217.1,305.2 for qualitative ions, m/z73.0 for quantitative ion were established. The linear relationship was good,the correlation coefficient was 0.9987 ,the detection limit was 9μg/100g, RSD were 7.997%, 2.515% ,2.529% （n =7） ,the method of recovery was between 88.5%-108.1% ,that all can satisfy the requirements of the sample testing.