蛇毒型神经生长因子对脑缺血再灌注损伤后大鼠脑组织细胞外信号调节激酶和核因子-κB表达的影响

Influencing of venom nerve growth factor on expressing of extracellular signal-regulated kinase and nuclear factor-κB in rat brain tissue after cerebral ischemia reperfusion injury

目的 观察蛇毒型神经生长因子（vNGF）对脑缺血再灌注损伤后大鼠神经重塑的影响.方法 雄性Wistar大鼠90只随机分成5组：vNGF-25u组、vNGF-50u组、vNGF-100u组、缺血再灌注（I/R）组和假手术（S）组,每组18只.第2、7、14天分批处死大鼠取脑组织提取总RNA,用荧光定量聚合酶链反应（PCR）检测细胞外信号调节激酶（ERK）mRNA和核因子（NF）-κB mRNA的表达.结果 （1）随着时间的延长,NF-κB mRNA的表达逐渐增加 同一时间,NF-κB mRNA在vNGF各组的表达高于I/R对照组 NF-κB mRNA的表达随着vNGF浓度的升高而增加.（2）随着时间的延长,ERK mRNA在各组的表达逐渐降低 术后同一时间,在I/R对照组的表达最高,vNGF各组次之.结论 脑缺血再灌注损伤后经侧脑室给予vNGF,可以通过正性调节NF-κB转导通路和负性调节ERK通路,从而促进神经再生和恢复神经缺损功能.

Objective To investigate the significance and mechanism of Intracerebroventricular injection viper venom nerve growth factor （vNGF） in rat neural plasticity after cerebral ischemia reperfusion injury. Methods Ninety wistar male rat were randomly assigned into vNGF-25u group （ n = 18）, vNGF-50u group（n = 18）, vNGF-100u group（n = 18）, ischemia reperfusion （I/R） group（n = 18） and sham operated（s） group. The expression of extracellular signal-regulated kinase （ERK） mRNA and nuclear factor-κB （NF-κB） mRNA in rat brain tissues which were collection at 2, 7, 14 days after surgery were evaluated by the Real-time polymerase chain reaction （PCR）. Results （ 1 ） The expression NF-κB mRNA began to increase after surgery, while ERK mRNA decreased. At the same time, expression of NF-κB mRNA in the vNGF groups more than in I/R group. The more vNGF were injected, the more NF-κB mRNA were expressed. （2） The expression of ERK mRNA in I/R control group more than other groups.Conclusion The vNGF could accelerate neural plasticity and restore neurofunctional defect through up-regulated the expression of NF-κB and down-regulated the expression of ERK.