吗啡对胃癌细胞生物学性状的影响

Effects of morphine on biological characters of human gastric carcinoma cell line

目的 观察吗啡对人胃癌MGC-803细胞生物学性状的影响.方法 将吗啡加入细胞培养液中使吗啡浓度分别稀释至0.1、10、1000 μmol/L,分别与胃癌MGC-803细胞一同孵育,应用噻唑蓝（MTT）比色法绘制12、24、36、48、60、72h的细胞生长曲线并计算细胞增殖率,7d后应用克隆形成实验检测MGC-803细胞生长增殖的变化 应用流式细胞仪检测MGC-803细胞周期和细胞凋亡的变化,用透射电子显微镜观察MGC-803细胞超微结构的变化.结果 吗啡使胃癌细胞生长缓慢,0.1 μmol/L吗啡组、10 μmol/L吗啡组、1000 μmol/L吗啡组细胞增殖率分别为对照组的（81.89±6.44）%、（78.52±4.68）%和（78.53±5.68）%（P＜0.05）,3组细胞的克隆形成率分别为（0.76±0.18）%、（0.72±0.17）%和（0.56±0.18）%,低于对照组的克隆形成率（1.19±0.29）%（P＜0.05） 与对照组比较,各吗啡组细胞周期G2/M期比例上升,G0/G1期和S期比例下降（P＜0.05） 0.1μmol/L吗啡组、10 μmol/L吗啡组、1000 μmol/L吗啡组细胞凋亡率分别为（17.20±2.95）%、（19.13±3.86）%、（24.38 ±4.94）%,明显高于对照组的凋亡率（11.40±2.86）%（P＜0.05） 透射电镜显示吗啡使胃癌细胞出现明显的凋亡表现.结论 吗啡抑制胃癌MGC-803细胞的生长增殖,促进胃癌细胞的凋亡.

Objective To explore the effects of morphine on biological characters of human gastric carcinoma cell line MGC-803. Methods MGC-803 cells were incubated with 0.1,10 or 1000 μmol/L morphine. The viability of cells was determined by MTT assay at 12, 24, 36, 48, 60, 72 h after being incubated with morphine and the proliferation of cells was measured by colony formation assay 7 days later.The cell cycle progression and apoptosis were assessed by flow cytometry. The ultrastructural changes of MGC-803 cells were observed under the transmission electron microscopy. Results The proliferation of MGC-803 cells was inhibited after incubation with morphine. Clones of gastric carcinoma cell line MGC-803 in morphine groups were fewer than those in control group. In morphine groups, the proportion of cells in G2/M was higher than that in control group. In morphine groups, apoptotic rate of MGC-803 cells was higher than in control group. Moreover, apoptotic bodies were observed in morphine groups. Conclusion Morphine can effectively inhibit growth and proliferation of gastric cancer cells and may be used as a potent therapy for gastric cancer.