检测尿酸和葡萄糖电极式试纸条的研制

The design of an electrochemical strip for uric acid and glucose assay

根据双电极系统中铁氰化钾的电子媒介新模式原理研制一种电极式印制型尿酸和葡萄糖试条。试条由完全对等的丝网印制双碳电极组成,测定范围宽(-0.6～0.6V),可根据检测物浓度和干扰情况选择测定电位和灵敏度。用尿酸和葡萄糖试纸条测试35例病人血清样品中尿酸和葡萄糖的含量,并与医院常规分析方法的测试结果对比,经配对T检验,双侧检验的概率P分别为0.427和0.079,测定结果差异无统计学意义(P>0.05)。铁氰化钾作为电子媒介体,其电子媒介作用是通过其转化成亚铁氰化钾来实现的,任何可导致上述转化发生的物质都可以借助铁氰化钾为电子媒介来测定,合理使用媒介体可以达到检测高过电位物质或放大检测信号等目的。

A disposal screen-printable uric acid and glucose electrode strip were fabricated based on the principle of ferricyanide-mediated electron transfer as the electron mediator, in two-electrode system. Both strips exhibit excellent linear responses to detecting substance in a wide measurement potential, -0.6 -0.6 V, which be adjusted at will according to concentration of detecting substance and level of interference. Statistical analysis shows the result of detection by strip and conventional method have preferable relativity.Thirty-five artificial samples of glucose or uric acid were taken and the contents of glucose and uric acid were tested using the proposed glucose and uric acid strips and the results were compared with that of routine analysis methods in hospital. It was evident that statistical analysis by Paired-samples T-Test showed the result of detection by the amperometric method gives the result of no significant differences （P was 0.427 and 0.079）. Potassium ferricyanide acts as an electron mediator via the reductive reaction to potassium ferroeyanide. Consequently, the substance which could be oxidized by potassium ferricyanide can be assayed by the amperometric method with potassium ferricyanide mediator. During the process, the analyte with high overpotential could be determined and the current signal could be enhanced.