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兔神经生长因子基因序列分析和真核表达载体的构建 被引量:1

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摘要 体外扩增神经生长因子(NGF)cDNA序列进行同源性分析,并构建其真核表达载体。序列分析显示兔NGF基因全长729bp,编码242个氨基酸,分子量为27.18KD,兔NGF核苷酸序列和氨基酸序列人源NGF的同源性分别是87.3%和77.3%。将兔NGF基因与真核表达载体pEGFP-N1连接,利用Western-Blot检测证实兔NGF在BHK-21细胞成功表达。真核表达载体pEGFP-N1-NGF的成功构建将为从分子水平开展对NGF的相关研究奠定基础。
出处 《中国社区医师(医学专业)》 2010年第28期6-8,共3页
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