Caspase-3和P—gP在原发性肝细胞癌中的表达和相关性研究

Expression of Caspase-3 and P-gp in hepatoceUular carcinoma and their relationship

目的通过检测原发性肝细胞癌及其癌旁组织中Caspase-3和P-糖蛋白（P—gp）的表达，了解两者与原发性肝细胞癌临床病理特征之间的关系及两者的相关性，为原发性肝细胞癌的治疗提供一定的理论依据。方法采用免疫组织化学Envision^TM二步法检测50例原发性肝细胞癌及其癌旁组织中Caspase-3和P—gp的表达，并结合患者的临床病理特征、随访资料进行分析。结果Caspase-3在原发性肝细胞癌组织中的表达低于癌旁组织（P〈0．05）；Caspase-3在原发性肝细胞癌组织中的表达与Edmonson病理分级、有无包膜有关（P〈0．05）。P—gP在原发性肝细胞癌组织中的表达高于癌旁组织（P〈0.05）；肝癌组织中P—gp的表达与Edmonson病理分级、是否有肝硬化有关（P〈0．05）。Caspase-3和P-gP的表达呈负相关（r=-0．600，P=0．000）。Caspase-3阳性表达的患者预后较好，P—gP阴性表达的患者预后也较好。结论Caspase-3可能促进原发性肝细胞癌细胞凋亡，抑制原发性肝细胞癌的发生；P-gP与原发性肝细胞癌的耐药性有关，还可能具有抗凋亡作用。Caspase-3低表达或失表达可能与原发性肝细胞癌细胞凋亡抵抗和多药耐药有关。

Objective To measure the expression of Caspase-3 and P-gp in hepatocellular carcinoma （HCC） and adjacent tissues, and to examine these two proteins＇ relationships with HCC clinical and pathological characteristics and the associations between the two proteins. Methods The expression of Caspase-3 and P-gp were measured in 50 HCC tissues and adjacent tissues by EnvisionTM immunohistochemistry. HCC clinic and pathological characteristics and follow-up data were also collected and analyzed. Results The expression of Caspase-3 in HCC tissues was significantly lower than that in adjacent tissues（ P 〈 0. 05 ）. The expression of Caspase-3 in HCC tissues was significantly associated with HCC＇s Edmonson grade and the presence of hepatitis envelope （P 〈 0.05）. On the other hand, the expression of P-gp in HCC tissues was significantly higher than that in adjacent tissues （P 〈 0.05 ）. P-gp expression in HCC tissues was significantly correlated with HCC＇s Edmonson grade and the presence of cirrhosis（P 〈 0.05 ）. The expression of Caspase-3 and P-gp was negatively correlated （ r = - 0.600,P =0. 000）. A better prognosis was observed among HCC patients with either positive Caspase-3 expression or negative P-gp expression（P 〈 0.05 ）. Conclusion Caspase-3 may promote apoptosis and inhibit the development of HCC, while P-gp may have an anti-apoptosis function and play a role in HCC＇s drug resistance. P-gp can inhibit the function of Caspase-3 and an absence or low expression of Caspase-3 may be related to apoptosis resistance and multidrug resistance.