摘要
采用HPLC-ELSD法测定绞股蓝中绞股蓝皂苷A的含量。色谱柱为HibarC18(250×4.6mm,5μm),流动相为乙腈-水梯度洗脱,流速0.5mL/min,柱温为20℃,绞股蓝皂苷A在0.0425—1.995mg/mL范围内线性关系良好(r2=0.9962),并通过正交法优化其提取工艺。该方法简便、有效,为绞股蓝的质量控制提供一种准确可行的检测方法。
Gypenoside A in Gynostemma pentaphyllum（Thunb.）Makino was determined by HPLC-ELSD.The separation was performed on Hibar C18 column（250×4.6mm,5μm）,by gradient elution using acetonitrile-H2O as the mobile phase at the flow rate of 0.5mL/min,and the temperature of column was 20℃.The linear range of gypenoside A was 0.0425—1.995mg/mL（r2=0.9962）.Also,the extraction was studied by the orthogonal experiment.This method was simple,effective and feasible,and can be used to control the quality of Gynostemma pentaphyllum（Thunb.）Makino.
出处
《光谱实验室》
CAS
CSCD
北大核心
2010年第5期1734-1737,共4页
Chinese Journal of Spectroscopy Laboratory
基金
"十一五"科技支撑计划项目(20060612)
