硫辛酸对围产期奶牛氧化状态的影响

Effect of α-lipoic acid on oxidative status in the transition cow

选择产前约20d的干乳期奶牛48头,分为4组,Ⅰ组为对照组,Ⅱ、Ⅲ、Ⅳ组分别每天补饲硫辛酸3、5、8g/头,连续50d,测定了血清谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的动态变化。结果表明,Ⅲ和Ⅳ组血清GSH-Px活性第20天开始显著或极显著高于对照组(P<0.05或P<0.01);Ⅲ和Ⅳ组血清SOD活性第30天开始降低的幅度明显低于对照组(P<0.05);Ⅱ组血清CAT活性在第30天显著高于对照组(P<0.05),Ⅲ、Ⅳ组第30～50天均显著高于对照组(P<0.05);Ⅲ组血清MDA含量在第40天、Ⅳ组在第50天显著低于对照组(P<0.05)。补充硫辛酸可明显改善围产期奶牛的抗氧化能力。

Forty-eight Holstein dairy cows at 20 dbefore calving were divided into four groups fed with α-lipoic acid （0,3,5or 8g/d）for 50 days,respectively.Serum activities of glutathione peroxidase（GSH-Px）,superoxide dismutase（SOD）and catalase（CAT）,and content of malondialdehyde（MDA）were measured.Serum GSH-Px,SOD activities were significantly higher at 20 dor 40 to 50 din groupⅢandⅣthan those of control group,respectively （P〈0.05or P〈0.01）.Serum CAT activities were significantly higher at 30 din groupⅡand 30,40 and 50 din groupⅢandⅣ when compared with control group（P〈0.05）.Serum MDA concentrations markedly reduced at 40 d in groupⅢand 50 din groupⅣ when compared with control group（P〈0.05）.The results suggest thatα-lipoic acid supplementation can improve antioxidant capacity in the transition cow.