反向核移植在水牛体细胞核移植中的初步研究

Primary study on buffalo somatic cell reverse nuclear transfer

探讨了反向核移植技术应用在水牛体细胞核移植中的效果。结果显示,反向核移植可支持重构胚发育到囊胚,但其构建重构胚的效率极显著低于电融合法(54.0%vs75.1%,P<0.01),囊胚发育率也显著低于电融合法(6.9%vs15.9%,P<0.05)。

Methods of reverse nuclear transfer were studied. The embryos reconstructed by reverse nuclear transfer could develop to blastocyst. But the cleavage rate of and blastocyst rate of reconstructed embryos were significant lower than that of electric fusion nuclear transfer （54.0% vs 75.1G ,P〈0.01 ; 6.9% vs 15.9G ,P〈0.05）.