沙葱中黄酮类化合物对小鼠非特异性免疫机能影响的研究

Studies on flavonoids from allium mongolium regel effects to non-specific immunity function in mice

通过分离纯化沙葱中的黄酮类化合物,以昆明种小白鼠为动物模型,研究饲喂沙葱黄酮对小鼠非特异性免疫机能的影响。试验应用纯昆明种小白鼠480只,体重(20±2)g,随机分为4组,每组120只(雌雄各半),分笼饲养。第1组为对照组,第2组为添加75.36mg(/kgBW·d)沙葱组,第3组为添加75.36mg(/kgBW·d)75%乙醇提取的沙葱总黄酮粗提物组,根据预试验的结果第4组为添加75.36mg(/kgBW·d)30%乙醇洗脱纯化后沙葱黄酮添加组,预饲期5d,正式试验期14d,正式试验期内2次宰杀采血并分离血清,剖离肝脏、胸腺和脾脏称重。试验结果显示,试验各组的脾脏指数高于对照组,但差异不显著(P>0.05),沙葱黄酮纯化组胸腺指数显著高于其它各组(P<0.05);沙葱黄酮纯化组的血清ACP含量显著高于其它各组(P<0.05),试验各组肝脏ACP含量显著高于对照组(P<0.05);试验各组碳廓清指数高于对照组,但差异不显著(P>0.05),沙葱黄酮纯化组的吞噬指数、血清溶菌酶含量显著高于其它各组(P<0.05)。沙葱黄酮纯化组能提高免疫器官指数、血液和肝脏的ACP和溶菌酶的含量,从而增强吞噬细胞消化和降解异物的能力,并且沙葱黄酮纯化组能显著提高小鼠的碳廓清指数(κ)和吞噬系数(α),因此,沙葱黄酮在增强机体非特异性免疫机能方面发挥着重要作用。

Is this paper, Kunming mice were used to study the effect of flavonoids separated and purified from allium mongolium regel on non-specific immunity function in body. 480 [weight （20±2） g] pureblooded Kongming mice, which were divided randomly into four groups and each group 120 （half female and half male）, were raised in different cages which were named control group, 75.36mg/kg. BW. d Allium mongolium Regel supplementation group,75.36 mg/（kg BW ·d） flavonoid extract supplementation group,75.36 mg/（kg BW·d） flavonoids purification supplementation group. Preparatory experimental period lasted for 5 clays and the formal experimental period lasted for 14 days. After two slaughters being done during the formal experimental period, two slaughter experiment were conducted,the blood was collected and serum was isolated ,and live thymus and spleen was dissected and weighed. The result show that, the spleen index of experiment groups were higher than that of control group, but the difference was not significant （P〉0.05）.The thymus index of flavonoids purification group was higher than that of other groups （P〈0.05）.The content of ACP in serum of flavonoids purification group was higher than that of other groups （P〈0.05）; The carbon clearance index of experiment groups was higher than that of control group, but the difference was not significant （P〉0.05）.The phagocytic index and lysozyme content in serum of flavonoids purification group were higher than other groups（P〈0.05）.