血管内皮细胞生长因子对小鼠触须毛囊体外培养的研究

Study on vascular endothelial growth factor(VEGF)in the culture of mouse vibrissa follicles in vitro

ＶＥＧＦ（血管内皮细胞生长因子）是新近发现于毛囊的生长因子。我们应用新近建立的Ｃ５７ＢＬ／６Ｊ小鼠触须毛囊体外培养模型，对ＶＥＧＦ促毛囊生长的生物学特性进行研究。结果发现ＶＥＧＦ有明显促毛发生长作用。在培养的第２天，ＶＥＧＦ组毛发生长长度就明显优于阴性对照组。ＶＥＧＦ促毛囊生长作用随其浓度增高而增强，当浓度为１０ｎｇ／ｍｌ时促生长作用达高峰。ＶＥＧＦ和ＨＧＦ（肝细胞生长因子）联合培养对小鼠触须毛囊体外培养的结果显示ＶＥＧＦ与ＨＧＦ促毛囊生长有协同作用，但ＶＥＧＦ促毛发生长的作用在培养的前３天较明显，而ＨＧＦ促毛发生长作用在培养６天以后明显优于ＶＥＧＦ。

VEGF was newly found in the hair follicle papilla Using hair follicle culture model of mouse vibrissa we investigated the biologic effects of VEGF on hair growth in vitro The results showed that the hair growth in VEGF group was significantly longer than that in control group( P < 0 05) This growth stimulating of VEGF growth was the concentration dependent 10ng/mL VEGF showed a maximum stimulating effect on hair growth The culture of combination of VEGF and HGF indicated that these two growth factors had cooperative effect on hair growth