摘要
目的探讨银杏叶提取物对家兔动脉粥样硬化形成的影响以及作用机制。方法 23只新西兰大白兔随机分为对照组、假手术组、粥样硬化模型组(AS)、银杏叶提取物组(GBE)。采用高脂饮食联合球囊损伤复制家兔动脉粥样硬化模型,药物干预4周,抽血检测各组血脂水平;病理切片HE染色;免疫组织化学法和半定量逆转录-聚合酶链式反应(RT-PCR)法检测单核细胞趋化蛋白-1(MCP-1)蛋白和mRNA表达。结果银杏叶提取物(100mg/kg)对高脂血症无明显抑制作用(P>0.05),但可明显抑制斑块形成(P<0.05),下调MCP-1mRNA和蛋白表达(P<0.05)。结论银杏叶提取物对动脉粥样硬化病变有一定的抑制作用,这种作用可能与下调MCP-1的表达有关。
Objective To investigate the effect of Ginkgo Biloba extract（GBE）on the atherosclerosis in common carotid artery of rabbits and to explore its mechanism.Methods Twenty-three new Zealand male rabbits were randomly divided into control group,sham-operation group,atherosclerosis group（AS）,and GBE group.Rabbit models of atherosclerosis were induced by high-fat diet combined with balloon injury.The medication lasted 4 weeks.Serum lipid levels in all 4 groups were detected.The carotid artery segments were harvested for histomorphological observation.Monocyte chemoattractant protein-1（MCP-1）protein level were tested though immunohistochemical assay.Expression of MCP-1 gene was detected by RT-PCR.Results GBE（100 mg/kg/d）could not lower serum lipid level（P 0.05）,but significantly inhibited the formation of atherosclerotic plaque and reduced the expression of MCP-1（P 0.05）.Conclusion GBE can inhibit MCP-1 expression in common carotid artery of atherosclerotic rabbits,which may play an important role in preventing atherosclerosis.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第5期487-490,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
贵州省优秀科技教育人才省长专项资金项目(2005225号)
