整合素β_3和细胞内粘附分子在子宫内膜异位症在位与异位内膜组织中的表达

Expression of Integrin β 3 and Intracellular Adhesion Molecule in Endometrium and Endometriotic Tissue

目的探讨整合素β３和细胞内粘附分子（ＩＣＡＭ）与子宫内膜异位症的关系。方法收集子宫内膜异位症患者的子宫内膜（１５例）、异位病灶组织（１５例）及非子宫内膜异位症患者（３０例，对照组）的子宫内膜，分３批对这些组织进行整合素β３和ＩＣＡＭ的蛋白免疫印记法测定，并用ＶＤＳ扫描对实验结果进行半定量。每批结果均用方差分析和ｔ检验进行统计处理。结果３批结果趋势相同，子宫内膜异位症患者子宫内膜的整合素β３和ＩＣＡＭ蛋白表达量均明显低于对照组同期子宫内膜的表达量。而异位病灶组织整合素β３的表达较对照组明显降低，ＩＣＡＭ表达却明显高于对照组。结论子宫内膜异位症患者从子宫内膜到异位病灶，整合素β３和ＩＣＡＭ均有明显的变化，这可能与子宫内膜异位症发生、发展和不孕有关。

Objective To study the relationship between integrin β 3 ,intracellular adhesion molecule(ICAM) and endometriosis. Methods The expression of integrin β 3 and ICAM protein were determined in 3 batches by immunoblotting method in 15 endometrium and 15 endometriotic tissues of patients with endometriosis and 30 endometrium from non endometriosis subjects during the same menstrual phase. The results were scanned and semi quantified, and then analysed by F test and t test. Results The results from 3 measurements showed the same trend .Endometrium from patients with endometriosis expressed significantly fewer integrin β 3 and ICAM protein than those without endometriosis. On the other hand , the expression of integrin β 3 in endometriotic tissue was significantly lower while the ICAM expression was significantly higher than those in controls. Conclusions In patients with endometriosis, integrin β 3 and ICAM expression changed significantly from eutopic to ectopic endometrium which may be related to the development of endometriosis and infertility.