流式细胞术对冠心病患者血小板表面活化标志蛋白的研究

Flow cytometry for assessing plateletactivation in coronary heartdisease

目的 :探讨流式细胞术 (FCM)测定的血小板活化指标与冠心病 (CHD)的关系。方法 :利用 FCM和单克隆抗体测定 31例稳定型心绞痛 (SAP组 )、65例不稳定型心绞痛 (UAP组 )和 2 5例急性心肌梗死 (AMI组 )患者的外周血中血小板糖蛋白 CD62 P、CD63和凝血酶敏感蛋白 (TSP)的阳性表达率 ,并与 39例正常人作对照。结果 :FCM可简单、迅速地检测血小板的活化功能 ,CHD患者 CD62 P、CD63和 TSP的阳性表达率均显著高于对照组 (P <0 .0 5)。在 CHD患者中 ,UAP组和AMI组的 CD62 P、CD63和 TSP的表达率均显著高于 SAP组 (P <0 .0 5) ,AMI组又高于 UAP组。结论 :CHD患者血小板活化是导致血小板功能亢进的原因 ,并参与了 CHD尤其是 UAP和AMI的病理过程 ;FCM是测定血小板活化功能既准确又可靠的方法之一。

Objective:To assess the ability of flow cytometry( FCM) and fluorescently labeled antibodies to detect platelet activation in patients with coronary heart disease( CHD) .Method:The alpha- granule membrane protein( CD62 P) ,lysosomal GP5 3( CD63) and TSP were measured from whole- blood samples in 12 1patients with CHD,which including 31stable angina pectoris( SAP) ,65 unstable angina pectoris( UAP) and2 5 acute myocardiol infarction( AMI) and in39normal controls using FCM and monoclonal antibody.Results:There were increases in platelet CD62 P,CD63,TSP expression in CHD than those in controls( P <0 .0 5 ) .CD62 P,CD63and TSP in the patients with UAP and AMIwere significantly higher than those in SAP ( P <0 .0 5 ) .Conclusion:These findings suggest that platelet activation relate to pathophysiological process of CHD and FCM provides an accurate and reliable technique for measuring the platelet activation.