IFN-α逆转白血病细胞耐药性的实验研究

Reversing effects of interferon α on drug resistance of bone marrow cells in leukemia

目的：了解干扰素－α（ＩＦＮ－α）逆转白血病细胞多药耐药性（ＭＤＲ）的作用和可能机制。方法：采用链亲和素－胶体金原位杂交（ＳＡＧ－ＩＳＨ）检测了ＩＦＮ－α孵育前后的５２例白血病患者骨髓细胞的多药耐药基因（ＭＤＲ１）表达，用荧光分光光度法测定了ＩＦＮ－α孵育前后的５２例骨髓细胞内柔红霉素（ＤＮＲ）浓度。结果：全部５２例患者中２４例（４６．２％）ＭＤＲ１阳性，初治组与复发难治组相比相差显著。ＭＤＲ１阳性者骨髓细胞内柔红霉素浓度与阴性者相差显著。ＩＦＮ－α孵育后的ＭＤＲ１阳性率与孵育前相差不显著，但经ＩＦＮ－α孵育前后的ＭＤＲ１阳性者的骨髓细胞内ＤＮＲ浓度相差显著，阴性者亦然。结论：ＩＦＮ－α能增加白血病细胞内ＤＮＲ浓度，其逆转ＭＤＲ环节不在ＭＤＲ１基因水平上。

Objective: To investigate the reversing effects of interferon α (IFN α) on drug resistance of bone marrow cells in leukemia and its possible mechanism. Methods: Before and after the bone marrow cells extracted from 50 cases of acute leukemia and 2 of chronic leukemia were incubated in the media with the addition of IFN α, MDR 1 mRNA expression and concentration of daunorubicin (DNR) in the cells were determined respectively with SGA ISH and respectrofluorimetry. Results: Twenty four (46.2%) out of all the 52 cases presented MDR 1 mRNA expression. There was significant difference in the concentration of DNR between MDR 1 positive and MDR 1 negative cases before and after incubation. In all the 52 cases, no significant difference was found between the concentration of DNR before the incubation and that after the incubation. Conclusion: IFN α can increase the concentration of DNR in the bone marrow cells in leukemia. The occurrence of MDR reversal is not on the level of MDR 1 but on other levels.