人酰基辅酶A:胆固醇酰基转移酶N端片段在大肠杆菌中的表达

Expression of human ACAT N terminal fragment in E. coli

目的：重组表达人ＡＣＡＴ的Ｎ端片段。方法：将编码ＡＣＡＴ氨基末端包含第一个跨膜区（１～１９６ａａ）的ｃＤＮＡ片段克隆至不同的大肠杆菌表达载体中，构建了多种大肠杆菌表达质粒，再转化至大肠杆菌中进行表达研究。结果和结论：在详细摸索表达条件的基础上，经温敏诱导，成功地在大肠杆菌ＡＲ６８中表达了Ｎ端融合ｐｒｏｔｅｉｎＡＢＣｄｏｍａｉｎ的人ＡＣＡＴ氨基末端包含第一个跨膜区的片段，表达量约２０ｍｇ／Ｌ菌液。为进一步表达完整的ＡＣＡＴ蛋白。

Objective: To express acyl coenzyme A: cholesterolacyl transferase (ACAT) N terminal containing the membrane anchoring region 1 (ACATn). Methods: The gene encoding the ACATn was cloned into the expression vector and transferred into E. coli. Results and Conclusion: The expressed product of ACATn fused with protein ABC domain and was detected in E.coli AR68.