高原RDS表面活性物质相关蛋白A、B、CmRNA表达的变化

Expression of pulmonary surfactant associated protein A, B and C mRNA in plateau ARDS *

目的：观察高原ＲＤＳ时ＳＰ－Ａ、ＳＰ－Ｂ和ＳＰ－ＣｍＲＮＡ表达与影响因素，了解其变化情况，并探讨其在急性肺损伤中的作用机制及意义。方法：采用高原ＲＤＳ动物模型，利用分子生物学技术观察肺组织肺泡Ⅱ型上皮细胞表面活性蛋白及其基因的表达。结果：ＳＰ－Ａ、ＳＰ－Ｂ和ＳＰ－Ｃ斑点杂交结果表明，油酸组ＳＰ－Ａ，ＳＰ－Ｂ和ＳＰ－ＣｍＲＮＡ表达的相对含量均显著低于对照组。油酸可抑制ＳＰ－Ａ，ＳＰ－Ｂ和ＳＰ－ＣｍＲＮＡ在高原ＲＤＳ中的表达。结论：高原ＲＤＳ后ＳＰ－Ａ、ＳＰ－Ｂ和ＳＰ－ＣｍＲＮＡ表达明显减低，油酸可抑制ＳＰ－Ａ，ＳＰ－Ｂ和ＳＰ－ＣｍＲＮＡ在高原ＲＤＳ中的表达。与油酸的直接毒性作用和油酸引起的肺水肿。

Objection: To study the expression and regulation of pulmonary surfactant associated protein A, B and C mRNA on oleic acid induced model of RDS of rats under acute hypoxia. Meanwhile, the mechanism of the changes was investigated. Methods: Rats were placed in hypobaric chamber for 12 h and then oleic acid was injected into the superior vena cava to induce RDS. The expression of pulmonary surfactant associated protein A, B and C mRNA was observed with the method of dot blot hybridization. Results: Oleic acid could inhibit the expression of surfactant associated proteins since SP A, SP B and SP C mRNA were expressed significantly lower in the group receiving loeic acid injection. Conclusion: Oleic acid can inhibit the expression of SP A, SP B and SP C mRNA in hypoxic rats with pulmonary injury.